ABC - Department of Animal Biotechnology - Ruminant genome biology group

Research Interests

Our research focuses on the development and application of modern animal biotechnology methods. We try to specify genetic markers causing bovine diseases. Our other aim is to develop new genome editing technologies to assist precision animal breeding.

Current Research Projects

Genetic markers of bovine diseases
Genome editing and transgenesis

 

Genetic markers of bovine diseases

The left displacement of abomasum (LDA) is a common disease in dairy cattle, despite the precise genetic basis is unclear. The only known genetic factor in connection of LDA is an SNP in the promoter region of the motilin gene. The mutation was connected to the LDA phenotype with association studies, but the functional analysis of the mutation is still missing. With the methods of biotechnology and genomics the project investigate on the effect of this SNP to the gene expression pattern. Besides we would like to find other genetic factors that influence the development of LDA disease.

This work is supported by (OTKA) National Research, Development and Innovation Office (NKFIH) PD 111964.

 

Genome editing and transgenesis

Genome editing is currently the state-of-the-art method in biotechnology. Using CRISPR/Cas9 system we are able to introduce targeted mutations in the genome which are equivalent to natural mutations. Genome editing offers dramatic advances in agricultural research. We use Sleeping Beauty mediated transgenesis to create animal models overexpressing some interest proteins. We adapt and develop genome editing tools for precision breeding in animal biotechnology following the 3R principles for animal research.

As a complex disease, LDA presumably depends on several genetic factors. There is also a pharmacological and evolutional interest if other gastrointestinal hormones and molecules can interact with the motilin receptor. A motilin knockout rabbit could clarify some undecided questions about the cross-interaction of motilin receptor and its putative ligands in vivo. The motilin receptor in rabbit is structurally and functionally similar to the bovine and human orthologue. As in rodent species the functional MLN and MLNR is absent, rabbit is the most convenient small laboratory animal for the modelling of MLNR. A rabbit line that lacks the motilin could give some answers may lead to better understanding of the gastrointestinal hypomotility diseases such as the LDA.

This work is supported by (OTKA) National Research, Development and Innovation Office (NKFIH) 124708.

 

Staff

Orsolya Ivett Hoffmann PhD
Munkakör: csoportvezető, tudományos főmunkatárs
Munkavégzés helye: 2100 Gödöllő, Szent-Györgyi Albert utca 4.
Telephone: +36 28526152
Fax: +36 28526101
E-mail: hoffmann.orsolya.ivett[at]abc.naik.hu
Bálint Biró
Munkakör: Önkéntes, tudományos segédmunkatárs
Munkavégzés helye: 2100 Gödöllő, Szent-Györgyi Albert utca 4.
Telephone: +36 28526253
E-mail: biro.balint[at]abc.naik.hu
Zoltán Gál
Munkakör: Önkéntes, tudományos segédmunkatárs
Munkavégzés helye: 2100 Gödöllő, Szent-Györgyi Albert utca 4.
Telephone: +36 28526253
Fax: +36 28526101
E-mail: gal.zoltan[at]abc.naik.hu
Nándor Lipták PhD
Munkakör: tudományos munkatárs
Munkavégzés helye: 2100 Gödöllő, Szent-Györgyi Albert utca 4.
Telephone: +36 28526253
Fax: +36 28526101
E-mail: liptak.nandor[at]abc.naik.hu

Publications

Publications (2010 – present)

 

A genomic landscape of mitochondrial DNA insertions in the pig nuclear genome provides evolutionary signatures of interspecies admixture
Schiavo, Giuseppina ; Hoffmann, Orsolya Ivett ; Ribani, Anisa ; Utzeri, Valerio Joe ; Ghionda, Marco Ciro ; Bertolini, Francesca ; Geraci, Claudia ; Bovo, Samuele ; Fontanesi, Luca, DNA RESEARCH 24 : 5 pp. 487-498. , 12 p. (2017), DOI URL ISSN: 1340-2838

A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid.
Tálas András, Péter Istvan Kulcsár, Nóra Weinhardt, Adrienn Borsy, Eszter Tóth, Kornélia Szebényi, Sarah Laura Krausz, Krisztina Huszár, István Vida, Ádám Sturm, Bianka Gordos, Orsolya Ivett Hoffmann, Petra Bencsura, Antal Nyeste, Zoltán Ligeti, Elfrieda Fodor, Ervin Welker, DNA RESEARCH 24 : 6 pp. 609-621. , 13 p. (2017)

Glomerulosclerosis in transgenic rabbits with ubiquitous Venus protein expression.
Lipták N, Hoffmann OI, Skoda G, Gócza E, Kerekes A, Bősze Z, Hiripi L. Acta Vet Hung. 2018 Jun;66(2):281-293. doi: 10.1556/004.2018.026.

Secretion of a recombinant protein without a signal peptide by the exocrine glands of transgenic rabbits.
Kerekes A, Hoffmann OI, Iski G, Lipták N, Gócza E, Kues WA, Bősze Z, Hiripi L. PLoS One. 2017 Oct 27;12(10):e0187214. doi: 10.1371/journal.pone.0187214. eCollection 2017.

Placenta-specific gene manipulation in rabbits.
Skoda G, Hoffmann OI, Gócza E, Bodrogi L, Kerekes A, Bösze Z, Hiripi L. J Biotechnol. 2017 Oct 10;259:86-90. doi: 10.1016/j.jbiotec.2017.07.037. Epub 2017 Aug 1.

Monitoring of Venus transgenic cell migration during pregnancy in non-transgenic rabbits.
Lipták N, Hoffmann OI, Kerekes A, Iski G, Ernszt D, Kvell K, Hiripi L, Bősze Z. Transgenic Res. 2017 Apr;26(2):291-299. doi: 10.1007/s11248-016-9994-9. Epub 2016 Nov 10.

Transposon-Based Reporter Marking Provides Functional Evidence for Intercellular Bridges in the Male Germline of Rabbits.
Hoffmann OI, Kerekes A, Lipták N, Hiripi L, Bodo S, Szaloki G, Klein S, Ivics Z, Kues WA, Bosze Z. PLoS One. 2016 May 5;11(5):e0154489. doi: 10.1371/journal.pone.0154489. eCollection 2016.

Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons.
Ivics Z, Hiripi L, Hoffmann OI, Mátés L, Yau TY, Bashir S, Zidek V, Landa V, Geurts A, Pravenec M, Rülicke T, Bösze Z, Izsvák Z. Nat Protoc. 2014 Apr;9(4):794-809. doi: 10.1038/nprot.2014.009. Epub 2014 Mar 13.

Germline transgenesis in rodents by pronuclear microinjection of Sleeping Beauty transposons.
Ivics Z, Mátés L, Yau TY, Landa V, Zidek V, Bashir S, Hoffmann OI, Hiripi L, Garrels W, Kues WA, Bösze Z, Geurts A, Pravenec M, Rülicke T, Izsvák Z. Nat Protoc. 2014 Apr;9(4):773-93. doi: 10.1038/nprot.2014.008. Epub 2014 Mar 13.

Low titer lentiviral transgenesis in rodents with simian immundeficiency virus vector.
Bender B, Hoffmann OI, Negre D, Kvell K, Bősze Z, Hiripi L. Biotechniques. 2013 Sep;55(3):137-40. doi: 10.2144/000114078.

Transposon-mediated transgenesis, transgenic rescue, and tissue-specific gene expression in rodents and rabbits.
Katter K, Geurts AM, Hoffmann O, Mátés L, Landa V, Hiripi L, Moreno C, Lazar J, Bashir S, Zidek V, Popova E, Jerchow B, Becker K, Devaraj A, Walter I, Grzybowksi M, Corbett M, Filho AR, Hodges MR, Bader M, Ivics Z, Jacob HJ, Pravenec M, Bosze Z, Rülicke T, Izsvák Z. FASEB J. 2013 Mar;27(3):930-41. doi: 10.1096/fj.12-205526. Epub 2012 Nov 29.

Characterization of the rabbit neonatal Fc receptor (FcRn) and analyzing the immunophenotype of the transgenic rabbits that overexpresses FcRn.
Catunda Lemos AP, Cervenak J, Bender B, Hoffmann OI, Baranyi M, Kerekes A, Farkas A, Bosze Z, Hiripi L, Kacskovics I. PLoS One. 2012;7(1):e28869. doi: 10.1371/journal.pone.0028869. Epub 2012 Jan 11.

Events

There are currently no upcoming events.